Published customer image:
Rat anti Mouse CD279 antibody, clone RMP1-30 (MCA2464) used to detect PD-1 (CD279) expression in mouse cells by flow cytometry.
Image caption:
Confirmation of PD-1 expression as a marker of CD8+CD122+ regulatory T-cells in the CNS of PLPmut mice. (A) Representative example of flow cytometric analysis of T-lymphocytes freshly isolated from brains of untreated PLPmut mice gated for CD8 and CD122 expression. The majority of the CNS-derived CD8+ T-cells are CD122- effector cells (red); only few cells are double-positive for CD8 and CD122 (green) and thus regulatory T-cells. (B) Representative example of flow cytometric analysis of gated CD8+CD122- (red) and CD8+CD122+ (green) T-lymphocytes from brains of untreated PLPmut mice analysed for PD-1-coexpression. Only CD8+CD122+ regulatory cells coexpress PD-1. Right histograms display corresponding counts for CD8+CD122- (red) and CD8+CD122+ cells (green) and their coexpression of PD-1. Appropriate isotype control counts are represented in grey

From: Groh J, Hörner M, Martini R.
Teriflunomide attenuates neuroinflammation-related neural damage in mice carrying human PLP1 mutations.
.
doi: 10.1186/s12974-018-1228-z.
This image is from an open access article distributed under terms of a.

Published customer image:
Rat anti Mouse CD279 antibody, clone RMP1-30 (MCA2464) used to detect PD-1 (CD279) expression in mouse cells by flow cytometry.
Image caption:
Confirmation of PD-1 expression as a marker of CD8+CD122+ regulatory T-cells in the CNS of PLPmut mice. (A) Representative example of flow cytometric analysis of T-lymphocytes freshly isolated from brains of untreated PLPmut mice gated for CD8 and CD122 expression. The majority of the CNS-derived CD8+ T-cells are CD122- effector cells (red); only few cells are double-positive for CD8 and CD122 (green) and thus regulatory T-cells. (B) Representative example of flow cytometric analysis of gated CD8+CD122- (red) and CD8+CD122+ (green) T-lymphocytes from brains of untreated PLPmut mice analysed for PD-1-coexpression. Only CD8+CD122+ regulatory cells coexpress PD-1. Right histograms display corresponding counts for CD8+CD122- (red) and CD8+CD122+ cells (green) and their coexpression of PD-1. Appropriate isotype control counts are represented in grey

From: Groh J, Hörner M, Martini R.
Teriflunomide attenuates neuroinflammation-related neural damage in mice carrying human PLP1 mutations.
J Neuroinflammation. 2018 Jul 3;15(1):194.
doi: 10.1186/s12974-018-1228-z.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.